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Introduction to the Agilent 8453 UV-VIS Spectrophotometer and ChemStation Software

Improve your efficiency and data quality!
Learn to use this instrument and its software effectively!

This two-day seminar provides a complete description of the Agilent 8453 UV-VIS spectrophotometer and the ChemStation operating software. Starting from the basic principles of absorbance spectrophotometry, the student will be shown how to get the instrument and samples ready for analysis, and what options are available for calibrating the instrument as well as analyzing samples. Both the standard and advanced features of the instrument and software will be discussed. Laboratory "hands-on " sessions with the instructor allow more time for interactive discussion, demonstration, and problem-solving (laboratory access to instrument required).

Student Profile:

Anyone responsible for routine operation and maintenance of the Agilent 8453 UV-VIS Spectrophotometer with ChemStation software. Both basic and advanced level users will benefit from the comprehensive content.

Students will learn about:

  • The organization of the ChemStation software
  • The basic features of the instrument and software
  • The advanced features of the software


Day 1

  • Why UV-VIS?
  • Basics of Absorbance Spectrophotometery
  • Introduction to 8453 Hardware
  • Good Measurement Practices
  • Introduction to ChemStation Software
  • Other Operating Hints
  • Basic operating procedures
  • Diagnostics and Troubleshooting
  • Basic Applications
  • Hands-On Laboratory Discussion and Demonstration (Time permitting)

Day 2

  • Using the Advanced Mode Features of the ChemStation
    • Getting Ready for Analysis
    • Data Processing Options
    • Single Data Analysis (Single Component Analysis)
    • Multiple Data Analysis
    • Confirmation Analysis
    • Multiple Component Analysis
    • Optimize
    • Automation
  • Special Features and Operating Modes
    • Dissolution
    • Kinetics
  • Hands-On Laboratory Discussion and Demonstration

Course Length: 2 days

Note: ChemStation® is a registered trademark of Agilent Technologies, Inc. This presentation was prepared by ACCTA, Inc. and has not been reviewed or endorsed by Agilent Technologies.

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This page last updated October, 2007.