Systematic HPLC Troubleshooting

Using HPLC but experiencing frequent problems or downtime? You are not alone. Many labs struggle to maintain efficient HPLC productivity.
Struggling with peak shape problems or changing retention times? There are many common reasons for these problems. Do you know what they are?
Not meeting productivity expectations because not all staff can solve their individual HPLC problems. Good training is your best lab resource. When everyone knows more, they can solve problems more quickly.
Having problems with QC performance? The most successful labs know the “Best Practices” that keep the lab running smoothly.

If you just need to get better at troubleshooting your HPLC system, then this one-day course is for you. No complex theory discussions, just lots of practical information for solving problems. Taught by a practicing chromatographer, this course highlights routine system operation and its role in preventing future problems. The discussion makes extensive use of case studies and examples to help students learn by examining real-world problems and solutions. We finish with the famous “LC Quiz,” a group problem-solving discussion where we explore selected problems and work through the steps necessary to solve each issue. It’s the students’ favorite.

Outline

  • A General Approach to Organized Troubleshooting
    • Why are we here?
    • How do we get started with troubleshooting?
  • General Troubleshooting Strategies
    • Troubleshooting concepts
  • How to Read a Chromatogram
    • Basic chromatography issues
    • Measuring instrumental and chromatographic parameters
  • Integration advice for poorly resolved peaks.
  • Troubleshooting analytical results.
  • Instrument Troubleshooting
    • Design-related problems in LC
    • Routine maintenance of mobile phases, pumps, columns, and detectors
    • Linking symptoms and solutions with the LC Troubleshooting Matrix
  • Best Practices in Chromatography
    • A practical guide to preventing future problems.
  • The LC Quiz

This topic is available either on-site or remotely. Find more details in the flyer.