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General Analytical

Significant Figures and Rounding (HTML)
Free!

This course is free! Do you want to see what our web-based training courses are like? This is your chance to learn how effective on-line learning can be. Significant figures is an important aspect whenever you are reporting numerical data. In this course we will review the common rules for significant figures and rounding, and discuss how these rules can be applied to common laboratory procedures.

Approximate Time Required:
15 minutes

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Students will learn:

  • The rules for determining significant figures in mathematical operations
  • How to estimate the number of significant figures associated with certain laboratory procedures
  • The proper way to round "5's" (many people are doing it wrong!)

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Laboratory Skills

Propagation of Errors/Preparation of Standards (HTML)
$49
This course introduces the concept of propagation of errors as it applies to the preparation of analytical standards. The student is presented with a simplified version of the concept, along with estimated error values for common laboratory procedures. From this information, the student will learn how to calculate the total error for a given analytical procedure. Finally, different dilution schemes (parallel, serial, and combined) will be introduced and discussed. Review questions are provided at the end of the course to help assess your understanding of these concepts.

Approximate Time Required:
1 hour

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Students will learn:

  • Types of errors in laboratory procedures.
  • How errors are combined in consecutive procedures.
  • How these can errors be estimated.
  • How to select dilution schemes (parallel, serial, combined).

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Using Pipetting Instruments (HTML)
$29
This course provides an introduction to the use of manual pipetting instruments. General design features are discussed for air displacement and positive displacement models. The student will learn the assumptions that must be satisfied to properly use these instruments. Finally, common problems and their solutions will be discussed. General instructions for calibration are also provided.

Approximate Time Required:
45 minutes*

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Students will learn:

  • Key design features of pipetting instruments.
  • The nine critical operating assumptions that must be satisfied to get the best accuracy.
  • Common problems and how to avoid them.
  • How to calibrate pipetting instruments.

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*Note: this course includes video clips that illustrate proper techniques. The time required to complete the course may be longer if you have a slower internet connection.

Chromatography

Extra-Column Effects Calculator (HTML)
Free!

This tool is free! Extra-column effects add to the observed peak width (and decrease the number of theoretical plates). (This topic is discussed in detail in the on-line course on Understanding Modern LC Systems.) This calculator lets you estimate the magnitude of these effects. The user can enter values for the following parameters:

  • Column efficiency (number of theoretical plates)
  • Connecting tubing internal diameter
  • Connecting tubing length
  • Detector flow cell volume
  • Detector flow cell path length
  • Injection volume

The observed efficiency (number of theoretical plates) is then displayed, along with some other similar calculations. Using this tool will help you get the best performance from your LC system.

Approximate Time Required:
10 minutes

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Students will learn:

  • The relative impact of changes in connecting tubing, detector flow cell, and injection volume.

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Introduction to the ChemStation (HTML)
Free!

This course is free! The ChemStation is a powerful program, but it's complexity can make it difficult to use. This brief introduction to the software will discuss:

  • Using the Configuration Editor to set up your LC or GC instruments
  • The ChemStation "View" concept
  • The contents of each "View"
  • ChemStation file name extensions

This course will help you understand how the software is organized. Once you understand the organization, it is easier to find the features you need. Note: this course covers the standard ChemStation interface for GC and LC instruments (not the GC-MS version). This course covers the ChemStation interface through version A.10.02.

This course complements our other ChemStation topics, and should be completed first.

Approximate Time Required:
20 - 30 minutes

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Students will learn:

  • How the ChemStation is organized, and where to find the many features.

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If you need more detailed assistance with your Agilent HPLC or GC,
visit our Agilent Help page.

 

Introduction to the ChemStation (Narrated)
Free!

This course is free! The ChemStation is a powerful program, but it's complexity can make it difficult to use. This brief introduction to the software will discuss:

  • The ChemStation "View" concept
  • The contents of each "View"

This course will help you understand how the software is organized. Once you understand the organization, it is easier to find the features you need. Note: this course covers the standard ChemStation interface for GC and LC instruments (not the GC-MS version). This course covers the ChemStation interface through version A.10.02.

This course complements our other ChemStation topics, and should be completed first.

This seminar includes an audio track with narrative comments about each slide.

Approximate Time Required:
17 minutes

Media Used:
Narrated file.

Narrated seminars were recorded using software from the netspoke.com web site. You must have this software before you can view this type of content. Click here to download the files.

Students will learn:

  • How the ChemStation is organized, and where to find the many features.

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Order Free Content
If you need more detailed assistance with your Agilent HPLC or GC,
visit our Agilent Help page.

 

Introduction to the LC ChemStation-Part 1 (PowerPoint)
$49 (Download)

The ChemStation is a powerful program, but it's complexity can make it difficult to use. This introduction to the software will discuss:

  • ChemStation 'Views'
  • Editing Methods
  • Setting Up and Running Samples
  • Single Runs and Sequences
  • Loading Signals

After payment you will be able to download this presentation as a PowerPoint file for your personal use. The file includes "Notes" which can be printed. The notes contain more information than the static HTML courses, but not as much as the narrated versions. This format is best for self-study, not group learning.

Note: this course covers the standard ChemStation interface for LC instruments through version A.10.02.

This course should be completed before Part 2.

Approximate Time Required:
1 - 2 hours

Media Used:
PowerPoint

Students will learn:

  • How the ChemStation is organized, and how to get the system ready for analysis.

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Order ChemStation Content
If you need more detailed assistance with your Agilent HPLC or GC,
visit our Agilent Help page.

 

Introduction to the LC ChemStation-Part 2 (PowerPoint)
$49 (Download)

The ChemStation is a powerful program, but it's complexity can make it difficult to use. This introduction to the software will discuss:

  • Optimizing Integration Settings
  • Setting Up and Using Calibration Tables
  • Reporting Options
  • Other Topics

After payment you will be able to download this presentation as a PowerPoint file for your personal use. The file includes "Notes" which can be printed. The notes contain more information than the static HTML courses, but not as much as the narrated versions. This format is best for self-study, not group learning.

Note: this course covers the standard ChemStation interface for LC instruments through version A.10.02.

This course is best when taken after completion of Part 1.

Approximate Time Required:
1 - 2 hours

Media Used:
PowerPoint

Students will learn:

  • How to use your system after setup.

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Order ChemStation Content
If you need more detailed assistance with your Agilent HPLC or GC,
visit our Agilent Help page.

 

Calibrating with the ChemStation (HTML)

$29

The ChemStation can automatically set up a calibration curve and report quantities for analyzed samples, but many analysts don't understand how to set up the system. You don't have to manually enter the peak areas into a spreadsheet. This course will show you:

  • How to set up the calibration table
  • How to select calibration options, like internal standards and peak qualifiers
  • How to use the Calibration Settings window
  • How to update the calibration using automatic recalibration

The "Introduction to the Chemstation" course is a recommended prerequisite to this course. Note: this course covers the standard ChemStation interface for GC and LC instruments (not the GC-MS version) through version A.10.02.

Approximate Time Required:
30 minutes

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Students will learn:

  • How to set up and use the calibration features of the ChemStation.

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Order ChemStation Content
If you need more detailed assistance with your Agilent HPLC or GC,
visit our Agilent Help page.

 

Understanding Modern LC Systems (HTML)
$49

This course will discuss several general properties of modern HPLC systems that have an impact on system behavior and performance. Topics include:

  • Solvent (mobile phase) characteristics
  • Extra-column effects
  • Flow through packed beds
  • Gradient programming
  • Small bore columns
  • Column selectivity

The discussion for each topic will focus on how these parameters affect the operation and performance of HPLC systems.

Approximate Time Required:
1 hour

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Students will learn:

  • Important operating features of modern HPLC systems.
  • Symptoms and problems associated with these operating features.
  • Solutions to these problems.

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Integrating Chromatographic Peaks (Narrated)

$49

This course provides a simple tutorial on how to properly integrate chromatographic peaks. Topics include:

  • Introduction to resolution and peak integration
  • What baseline options are available?
  • Should you use area or height?
  • Which baseline option is most accurate for poorly resolved peaks? Hear about the latest research on this topic.

This seminar includes an audio track with narrative comments about each slide.

Approximate Time Required:
40 minutes

Media Used:
Narrated file.

Narrated seminars were recorded using software from the netspoke.com web site. You must have this software before you can view this type of content. Click here to download the files.

Students will learn:

  • Which baseline options are available.
  • Which baseline options are most accurate for poorly resolved peaks.

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Register/Order/Access Your Content
All Courses
Free Content
Lab Skills Content
Chromatography Content
ChemStation Content


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