This company had a product that consisted of a small molecule and a peptide mixture, along with other, non-critical components. A method was needed to confirm the amounts of each component in the formulation.
An existing LC method suffered from the following problems:
A representative chromatogram is shown.
ACCTA, Inc. conducted a thorough study of numerous column options from our list of preferred phases, and then identified conditions that allowed the quantification of both the peptide and small molecule.
An efficient method was developed that easily separated the small molecule isomers and produced several characteristic peaks for the peptide mixture. The method even allowed quantification of a second peptide mixture that was present in other formulations.
The final procedure offered the following advantages:
See the difference in the chromatogram.