Case Study #4
LC Method Development for Purine Metabolites in Urine
These compounds are difficult to separate by reversed phase LC because they are water soluble and show little retention. In this method, we were able to use an aqueous normal phase (ANP) sytem (also known as HILIC). Gradient programming and wavelength switching were also necessary, but the results (shown below) allowed easy determination in urine using a relatively simple mobile phase system.
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This page last updated February, 2009.