Existing analytical methods for creatine (and
creatinine) are not optimized when compared to current HPLC technology.
The columns are too long, the mobile phase typically requires addition
of buffers and ion-pairing agents, and retention times are longer
than they should be. Such systems reduce the lifetime of your HPLC
components (all mobile phases with dissolved solids to this!), use
too much mobile phase (increasing purchase and disposal costs),
and make you wait for an answer.
We have developed a new method that eliminates
these problems.
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With this method you will:
- Reduce analysis cycle time, thus
increasing your analytical throughput and decreasing analytical
costs.
- Save money on mobile phase preparation
time and materials costs.
- Achieve better reproducibility due
to simplified preparation and operating procedures.
The method has the following characteristics:
- Uses a simple and inexpensive mobile
phase that does not require buffers or ion pairing agents.
- Uses a commercially available column
from a reputable manufacturer.
- Can be used on any modern HPLC equipped
with an absorbance detector operating at 210 nm.
- Is linear from 10 - 300 ug/mL (ppm)
as creatine monohydrate and creatinine hydrochloride. Lower concentrations
can also be detected.
- Has an analysis time of less than 2
minutes!
- Has been used successfully for hundreds
of analyses in our laboratory.
Our method includes the following information:
- Materials and Equipment
- Preparation of Standards
- Preparation of Mobile Phase
- Instrument Equilibration and Calibration
- Analysis Conditions
- Sample Preparation Suggestions
- Quality Control
- Calculations
- Performance Data (see table below)
Performance Data |
% RSD |
Parameter |
Creatine |
Creatinine |
Retention Time (1) |
1.19 % (n = 89) |
0.68 % (n = 84) |
Peak Area (2) |
0.18 % (n = 6) |
0.20 % (n = 6) |
(1) Value for all injections (standards and
samples) in a sequence of 89 injections.
(2) Value for 30 ug/mL standard injected throughout sequence (beginning,
end, and
every 20 injections during sequence).
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You can purchase the details of this method
using one of the following options:
- Purchase the method details and a license
to use this method, with installation and training by your own
staff.
- Cost: $49, Delivery: electronic
(pdf format).
- Payment: Purchase Order/Check or order
on-line.
- Purchase the method details and a license
to use this method, with on-site training at your facility by
our staff.
- Cost: Call for quote, Delivery: one
day of on-site installation and training.
- Payment: Purchase Order/Check
- Purchase the method details, a license
to use this method, and method development services to adapt the
method to your specific product(s).
- Cost: to be determined based on your
individual needs
- Payment: Purchase Order/Check
Analysis of your samples in our laboratory
is also possible. Please contact us
for more information, or to receive one free analysis of your sample
type.
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Why Pay For a Method?
Our expertise is developing and optimizing
methods. If you aren't in the business of method development, you
would never be able to develop a rugged method like this one for
under $1000. If you do method development, you will recognize the
value in having the basic operating parameters already identified.
We have done the work for you, and are offering
the method details for less than it would cost to develop the method
in your laboratory.
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